The Lab has generated and kept Drosophila trangenic stocks from previous publications , if you would like to obtain any of the lines please contact Dr Ko-Fan Chen directly.
Sleep24hour is a small MS-Excel spreadsheet calculator that was developed in Chen et al 2019. It allows you to conduct sleep analysis and plot sleep profile for one 24 hour day. This tool can be downloaded directly from this eLife data depository, and it generates the following parameters:
overall sleep profile: minutes of sleep per 30 minutes across 24 hours;
total sleep: total minutes of sleep during 24 hours of day
day sleep: total minutes of sleep during 12 hours of day;
night sleep: total minutes of sleep during 12 hours of night;
number of day sleep bout: the number of sleep bout during 12 hours of day
number of night sleep bout: the number of sleep bout during 12 hours of night
day average sleep bout length: day sleep minutes divided by total number of sleep bout during 12 hours of day;
night average sleep bout length: night sleep minutes divided by total number of sleep bout during 12 hours of night;
day waking activity: number of beam crosses divided by total wake minutes during 12 hours of day;
night waking activity: number of beam crosses divided by total wake minutes during 12 hours of night.
A ReadMe instruction is included in the Excel spreadsheet, but if you have any problem please contact Dr Ko-Fan Chen directly from the contact below.
If you find this tool useful please cite the following two key papers:
Hung, Y.C., Akhtar, M., Sattoju, N., Li, X., Head, S., Ollerenshaw, T., Siefer-Gaillardin, C., Arulchelvan, J., Warren, B. and Chen, K.F.* (2026). A day sleep promoting role of phototransduction in Drosophila melanogaster. Neurobiology of Sleep and Circadian Rhythms 100146. https://doi.org/10.1016/j.nbscr.2026.100146
Chen, K.-F.*, Lowe,. S, Lamaze, A., Krätschmer, P., Jepson, J.E.C. Neurocalcin regulates nighttime sleep and arousal in Drosophila. (2019) eLife, 8, e38114